This system combines the 4D-Nucleofector Core Unit with the versatile X Unit to deliver advanced non-viral transfection for a broad range of cell types, including hard-to-transfect primary cells, stem cells, and cell lines. The system delivers substrates directly into the cytoplasm and cell nucleus, achieving high transfection efficiency alongside excellent cell viability. The system supports various substrates, including plasmid DNA, mRNA, siRNA, peptides, and CRISPR/Cas9 ribonucleoprotein (RNP) complexes. The X Unit accommodates flexible throughput and cell volumes using two formats: standard 100 µL single Nucleocuvettes (for medium-to-high cell numbers, up to 2 × 10⁷ cells) and 16-well Nucleocuvette strips (20 µL format for low cell numbers, ideal for multiple conditions and optimization). The Core Unit features an intuitive touchscreen interface loaded with pre-optimized, validated protocols for hundreds of cell types.
For training sessions or other enquiries, please contact Dr. Alan Leung or Dr. Ryan Chow.
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