ThermoFisher Orbitrap Q Exactive Plus LC/MS

The ThermoFisher Orbitrap Q Exactive Plus liquid chromatography/mass spectrometer is a high-resolution mass spectrometer specialized for protein and peptide analysis. This instrument offers high mass accuracy for accurate mass measurement of peptides and intact proteins, tandem mass spectrometry function for detailed peptide sequencing, and online connection with Hydrogen/Deuterium Exchange (HDX) automation system for protein structural analysis. This instrument supports a wide range of applications, including characterization of intact proteins and their oligomers, study of protein modifications and bindings, and determination of changes in protein conformational and dynamic properties.

Specifications:

Liquid Chromatography: ThermoFisher Vanquish Flex UHPLC

Trajan Hydrogen/Deuterium Exchange Automation System
Mass Spectrometer: ThermoFisher Q Exactive Plus

Ionization modes: Electrospray ionization (ESI), Nano-ESI
Configuration: Quadrupole-Orbitrap
Acquisition modes: MS scan, Product ion scan (MS2), Data-dependent acquisition
Mass range: m/z 50 – 8,000
Mass resolution (FWHM at m/z 200)
- Maximum: 280,000
- Common: 70,000
Mass accuracy: 3 ppm with external calibration, 1 ppm with internal calibration

Software:

Xcalibur
BioPharma Finder
HDExaminer

Major Applications:

Characterization of intact proteins and their oligomers
Determination of protein bindings and modifications
Determination of protein bindings and modifications

Hourly rate

Internal users: $100
Collaborators: $200
External users: $500

Location

W711

Dr Patrick Wong

Scientific Officer

Dr. Pui-kin SO

Senior Scientific Officer

Y208
34008034
pui-kin.so@polyu.edu.hk