Zeiss Lightsheet 7 Microscope

Light sheet fluorescence microscopy (LSFM), also termed selective plane illumination microscopy (SPIM), is a planar illumination technique in which fluorescence excitation and detection are decoupled into separate optical pathways. With this novel optical design, illumination using a thin laser light sheet provides an inherent optical sectioning capability without the need to adopt a pinhole, which significantly improves the efficiency of photon collection and imaging speed without compromising image quality. LSFM allows researchers to examine live, intact animal models, ex vivo tissue explants, 3-dimensional cell cultures and organoids, etc., at unprecedented speed with virtually no photobleaching or photocytotoxicity.

Zeiss Lightsheet Z.1 Selective Plane Illumination Microscope

Specifications:

Single- or dual-side laser light sheet illumination
Scanned laser light sheet to produce homogenous illumination across the field-of-view (FOV)
Pivot scan option to reduce stripes commonly observed in LSFM images
Multiview imaging to acquire complimentary information from different angles
Tiling tool to acquire panoramic images covering a substantially larger FOV
Imaging chambers for live samples with temperature control
Imaging chambers for samples that have undergone tissue clearing
Supported tissue clearing techniques include CLARITY, CUBIC, iDISCO and BABB (with refractive indexes from 1.33 to 1.58)
Supported sample size up to 2×2×3 cm³ (when using a 5× detection objective)
Detection objectives:

EC Plan-NEOFLUAR 5×/0.16
EC Plan-NEOFLUAR 5x/0.16 foc
W Plan-APO 20×/1.0 DIC
W Plan-APO 40×/1.0 DIC
Clr Plan-NEOFLUAR 20×/1.0 Corr n_d=1.45
Clr Plan-NEOFLUAR 20x/1.0 Corr n_d=1.53

Illumination objectives:

LSFM 5×/0.1
LSFM 5×/0.1 foc
LSFM 10×/0.2
LSFM 10×/0.2 foc

Sample chambers:

Sample chamber set for 5× (compatible with EC Plan-NEOFLUAR 5×/0.16)
Sample chamber set for 5×/cleared samples with n_d=1.45 (compatible with EC Plan-NEOFLUAR 5×/0.16)
Lightsheet 7 sample chamber set for 5×/large cleared samples (compatible with EC Plan-NEOFLUAR 5x/0.16 foc)
Sample chamber set for 20×/aqueous samples with n_d=1.33 (compatible with W Plan-APO 20×/1.0 DIC)
Sample chamber set for 20×/cleared samples with n_d=1.45 (compatible with Clr Plan-NEOFLUAR 20×/1.0 Corr n_d=1.45)
Lightsheet 7 sample chamber set for 20×/cleared samples (compatible with Clr Plan-NEOFLUAR 20x/1.0 Corr n_d=1.53)
Sample chamber set for 40×/aqueous samples with n_d=1.33 (compatible with W Plan-APO 40×/1.0 DIC)

Usage rate

Internal users: $185
Collaborators: $370
External users: $925

Discount for long-duration bookings:

Applicable to consecutive bookings of 8 hours or longer
A 50% discount will be applied to any segments that fall within non-office hours (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
Price adjustments, if any, will be made after the completion of the imaging session
The ULS reserves the right to interpret these terms

Location

Y205

Staff-in-charge

Dr. Michael Yuen

Senior Scientific Officer

Dr. Alan Leung

Scientific Officer