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Multiphoton microscopy (MPM) is an advanced fluorescence imaging technique for generating high-resolution optical sections from thick biological samples. While laser scanning confocal microscopy (LSCM) utilises a pinhole to remove out-of-focus lights from the image, MPM restricts the excitation volume to the laser focus using a pulsed long-wavelength laser. As the focus of the laser is the only location where photons are dense enough to excite the fluorophores, optical sectioning can be achieved without a pinhole. On the other hand, the use of excitation laser light in the infrared range is particularly suitable for live fluorescence imaging, for it would only cause minimal photocytotoxicity to live tissues and cells, as well as photobleaching to fluorophores when compared to the use of visible lasers in LSCM. Furthermore, infrared laser light can penetrate animal tissues with much less light scattering, making deep tissue imaging possible. The laser with continuously tuneable wavelengths also allows researchers to examine a wider range of fluorescent probes with diverse excitation spectra. 

 

Discount for long-duration bookings:

  • Applicable to consecutive bookings of 8 hours or longer
  • A 50% discount will be applied to any segments that fall within non-office hours (i.e., from 6 pm to 9 am on weekdays, and the whole days on Saturdays, Sundays and public holidays)
  • Price adjustments, if any, will be made after the completion of the imaging session
  • The ULS reserves the right to interpret these terms

Consumables:

  • Marienfeld no. 1.5H 18 mm round coverglass ($148; 100 pcs)
  • MatTek 35 mm no. 1.5 glass bottom culture dish ($190; 10 pcs)
  • Nunc Lab-Tek II no. 1.5 8-well chambered coverglass ($435; 8 pcs)
  • ProLong Diamond antifade mountant ($372; 2 mL)
  • ProLong Live antifade reagent ($333; 1 mL)
  • EMS 16% paraformaldehyde ($116; 10 mL)

Staff-in-charge

Dr. Alan Leung

Scientific Officer

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