10X Genomics Chromium iX Single Cell Analysis System

Single-cell analysis is an emerging technology that is commonly employed in the study of genomics, transcriptomics, proteomics and metabolomics. Because of the heterogeneous properties among cell populations, some rare events are always masked by studying a bulk population of cells. The single-cell analysis method can help to unmask the average results of the bulk cell population analysis and the rare events, which are always very important to signaling pathways, can be discovered.

The 10x Genomics Chromium iX Single Cell Analysis System employs the NEXT GEM technology to partition cells or nuclei, and each of the partitions contains a bead that is coated with a unique oligonucleotide barcode sequence and functionalized sequences to capture molecules of interest, so that all fragments or information from the same cell or nucleus share a common barcode, giving a single-cell sample for downstream reactions to create short-read sequencing libraries.