Introduction:
Optical fiber recording system is a commonly used tool for detecting the fluorescence signal of neuronal population. It is characterized by recording the activity changes of specific neurons in specific behavioral paradigms through optical technology, and can observe the neural projection activities of animals in complex behaviors in real time. It is of great significance to study the function of specific neural circuits in different behavioral paradigms. With the emergence of new photogenetic neurotransmitter fluorescent probes, the application scope of the system has been further broadened, enabling it to record the dynamic changes of neurotransmitters (such as dopamine, acetylcholine, adenosine, etc.) in specific brain regions in real time during animal behavior. It has become an important tool for studying the changes of neurotransmitters in vivo.
Specifications:
- Main device: FPS-MO-405 / 470 / 560, integrated signal acquisition, digital synchronization and expansion interface module, integrated aluminum alloy fuselage.
- Light source type: LED light source, excitation light center wavelength 410 nm, 470 nm, 561 nm ; the excitation light power is controlled bysoftware. The optical power adjustment range is 0-100 μW, and the adjustment accuracy is 0.1 μW.
- Photosensitive element: CMOS chip, sampling frame rate 1-320 fps adjustable, adjustment accuracy 1 fps ; the exposure time is 1-100 ms, and the adjustment accuracy is 1 ms. The gain setting range is 0-24, and the adjustment accuracy is 1.
- Filter compatibility: Compatible with 635 nm, 589 nm light source, each channel can achieve the same point stimulation and recording at the same time.
- The number of sampling channels: the number of sampling channels is unlimited, the number of sampling channels can be customized, and the ROI name and area size of each channel can be customized.
- Interface type: 8 digital signal interfaces, each of which can be set as an input or output interface through supporting software; 8 analog signal input interface, can set the signal acquisition range of 0-30 kHz, set the accuracy of 1 Hz; 4 analog signal output interface; 2 trigger interfaces.
- Data import and mapping: multiple sets of data can be imported in batches for simultaneous analysis ; the △ F / F, Z-score, Heat-map vector diagram can be drawn and exported without the help of third-party software such as Matlab. It can be specified to delete any Trial data in the Heat-map, and the Z-score and △ F / F will be changed synchronously. The Y-axis ruler can be customized before exporting the image.
Consumables:
- The fiber optic cannula with Ø1.25mm ferrule
- The fiber optic cannula with Ø2.5mm ferrule
- Optical Fiber Patch Cable (mono, dual or multiple fiber cables) with a standard FC or SMA connector on one end for connecting a light source or fiber photometry system, and Ø1.25 mm or Ø 2.5 mm ferrule on the other end for connecting fiber optic cannula via a mating sleeve.
