Huygens Professional Image Analysis Software

Huygens Professional is a powerful image processing and analysis platform widely used in microscopy. It is specifically designed for the deconvolution, restoration, and visualization of complex, multi-dimensional fluorescence microscopy images, including confocal, STED, and widefield datasets. Its advanced algorithms significantly enhance image resolution and contrast, enabling researchers to obtain more accurate and quantitative information from their data. Notably, the Huygens MLE algorithms conserve intensity values, ensuring that deconvolution results remain quantifiable. Furthermore, the deconvolved images are much easier to analyze compared to the raw data.

Huygens image-Fig1

Major features of Huygens Professional include:

Increase the resolution in X, Y and Z: 1.5 to 3 times, increase the contrast/max signals 5-20 times
Removes the noise, corrects background efficiently
Automatic bleaching effects correction
Automatic deconvolution of Time Series
Automatic correction for Spherical Aberration
Automated deconvolution via Workflow Processor
Automatic Z-drift correction
Improve the object aspect ratio
Deconvolution wizard guides you through (multi) -channel deconvolution
Simulation of microscopic image formation.
Experimental distillation and theoretical calculation of Point Spread Functions

Huygens image-fig2

Microscope types supported:

Confocal, widefield (e.g. Nikon Ti-2), multi-photon, spinning-disk, STED, Array-detector, Light-sheet, Rescan, Visitech, Aurox, 4-pi, etc.

File formats supported:

Leica (.lif file); Zeiss (.czi, .lsm, .zvi file); Nikon (.nd2 file), Deltavion (.dv file), .ome file, .tiff file, Abberior (.obf file), .msr file, etc.

Huygens image-fig3