Introduction:
Thermo Scientific Orbitrap Fusion Lumos Mass Spectrometer is a high resolution mass spectrometer that is capable of collecting high quality MS and tandem MS data for both non-targeted and targeted analysis. The multiple fragmentation techniques (CID, HCD, ETD and EThcD) facilitate diverse applications in chemical and biological research such as accurate mass measurement, structural elucidation of interested analytes, quantitative proteomics, post-translational modifications (PTMs) analysis and lipidomics.
Specifications:
Thermo Scientific Vanquish Neo UHPLC
- Binary nano pump with continuous direct nano flow
- Flow rate range: 1nL/min – 100 µL/min
- Maximum pressure: 1,500 bar (21,750 psi)
Dionex UltiMate 3000 DGLC
- Dual-Gradient Binary pump with continuous direct flow
- Double the throughput with tandem UHPLC, boost chromatgraphic reolution with 2D-UHPLC, or automate sample preparation with online SPE-UHPLC.
- Flow rate range: 0.001 mL/min – 8.0 mL/min
- Column compartment: 10C – 75C
- Leverage orthogonal selectivity to increase sample coverage, extend quantitative dynamic range
Thermo Fisher Orbitrap Fusion Lumos Mass Spectrometer
- Ion Source:
- Electrospray ionization (ESI)
- nanoelectrospray ionization (nano ESI)
- Atmospheric pressure chemical ionization (APCI)
- Scan modes: MSn (n = 1-10)
- Fragmentation techniques in MS/MS:
- Collision induced dissociation (CID)
- High-energy collision dissociation (HCD)
- Electron transfer dissociation (ETD)
- Electron transfer and high-energy collision dissociation (EThcD)
- Mass analyzer:
- Linear ion trap MS
- Orbitrap MS
- Mass range:
- m/z 50-2,000
- m/z 200-4,000
- up to m/z 6,000 without precursor ion selection (MS only)
- Resolution: 500,000 FWHM at m/z 200
- Mass accuracy:
- Less than 3ppm RMS using external calibration
- Less than 1ppm RMS using internal calibration
Applications:
- Biomarker discovery
- Labeled and label-free quantitative proteomics
- Protein identification
- Top-down proteomics
- Native mass spectrometry
Software:
- Progenesis QI for Proteomics (QIP) with Mascot
- Proteome Discoverer 2.5 (for Proteomics)
- Spectronaut 20 (DIA Proteomics)
- PEAKS Studio 12.5 (for DDA/DIA/ de novo sequencing)
Note to users:
First-time users must be trained by equipment-in-charge before they can use the instrument independently.
Samples should be desalted and purified prior to MS analysis to enhance the detection sensitivity and minimize contamination.
Charging:
Direct infusion:
Internal users $100 / hr; Collaborators $200 / hr; External users please enquiry
LC/MS:
Internal users $300 / 4hr; Collaborators $600 / 4hr; External users please enquiry
Internal users $500 / 8hr; Collaborators $1000 / 8hr; External users please enquiry
Internal users $1000 / 24hr; Collaborators $2000 / 24hr; External users please enquiry
Booking:
Please visit our University Research Facility Management System (URFMS) website for registration, training request and booking arrangement.
Contact Information:
Location: W711, Block W, The Hong Kong Polytechnic University
Equipment-in-charge: Dr. Sirius TSE / Dr. Chi-Hang Chow (Office: TU503)
Email: sirius.tse@polyu.edu.hk/ chihang.chow@polyu.edu.hk
Telephone: 3400 8878/ 3400 8813
