The PacBio Sequel system is equipped with the third generation sequencer PacBio Sequel, utilizing the Single Molecule Real-Time (SMRT) sequencing technology that was launched in September 2015 by Pacific Biosciences in California, United States. Long read lengths are its signature. Such capability facilitates genome annotation and de novo assembly, especially with repetitive elements, as well as finishing draft genomes by filling in gaps and minimizing alignment errors, which is a challenge with second generation sequencers. Long read lengths also enable sequencing of full length transcripts and increases resolution of heterozygosity in diploid genomes via haplotype phasing.
Our facility also includes other equipment to ensure the quality of DNA prior to sequencing. These include: Covaris M220 focused-ultrasonicator, Agilent 2100 Bioanalyzer, Thermo Fisher Scientific Qubit 3.0 Fluorometer and NanoDrop 2000c, Sage Science BluePippin system, Applied Biosystems StepOnePlus RealTime PCR system and Veriti Thermal Cycler, Next Advance Bullet Blender, and Hoefer and Baygene gel electrophoresis systems.