Introduction:
Thermo Scientific Orbitrap Exploris Mass Spectrometer is a high resolution mass spectrometer that is capable of collecting high quality MS and tandem MS data for both non-targeted and targeted analysis. The fragmentation techniques of HCD facilitate MS/MS fragmentation which can apply in environmental analysis and metabolomic research.
Specifications:
Thermo Scientific TRACE 1600 Series GC
- Carrier gas: Helium
- Reagent gas: Methane (for CI)
- Split/Splitless injector (SSL)
- Oven Operation Temperature range: ambient+3°C to 450°C
- Oven Temperature set point resolution: 0.1°C
- Oven cool down: 450°C to 50°C in less than 4 minutes
- Split ratio: up to 12500:1
- Split flow: OFF or from 0-1250 mL/min in 0.1 mL/min increments
- Pressure range: 0-150 psi
- Injector Maximum temperature: 400°C
TriPlus RSH SMART Advanced Autosampler
Perform the change of the injection tool automatically for unattended sequence with different samplling techniques, and supporting the automation of multi-setep sample preparation workflows.
- Incubator/agitator
- Capacity 6 x 20 mL vials (compatible with 10 mL vials with adapters)
- 30-200°C temperature range
- 250-750 rpm agitation speed
- Headspace Sampling
- Compatible with 2, 10 and 20 mL vials
- Syringe temperature: OFF or 40°C to 150°C in 1°C steps
- Incubation oven: 30-200°C temperature range, in 1°C steps; 250-750 rpm agitation speed
- Incubation time: 0.1 to 600.0 min in 0.1 min increments
- Solid-phase microextraction (SPME)
- Incubation oven: 30-200°C temperature range, in 1°C steps; 250-750 rpm agitation speed
- Incubation time: 0.1 to 600.0 min in 0.1 min increments
Thermo Fisher Orbitrap Exploris Mass Spectrometer
- Ion Source:
- Electron ionization (EI)
- Chemical ionization (CI): Postiive Ion Chemical ionization (PCI) and Negative Ion Chemical Ionization (NCI)
- Scan modes: MSn (n = 1-10)
- Fragmentation techniques in MS/MS:
- High-energy collision dissociation (HCD)
- Mass analyzer:
- Orbitrap MS
- Mass range:
- m/z 30-3,000
- Resolution: 60,000 FWHM at m/z 200
- Scan rate: 40 Hz at resolution setting 7,500 at m/z 200
- Mass accuracy:
- Less than 3ppm RMS using external calibration
- Less than 1ppm RMS using internal calibration
Applications:
- Environmental and Food Analysis
- Metabolomics
Software:
- Compound Discoverer (For Non-Target Analysis)
- TraceFinder (For Quantitative Analysis)
- Progenesis QI (Waters)
Note to users:
First-time users must be trained by equipment-in-charge before they can use the instrument independently.
Samples should be desalted and purified prior to MS analysis to enhance the detection sensitivity and minimize contamination.
Booking:
Please visit our University Research Facility Management System (URFMS) website for registration, training request and booking arrangement.
Contact Information:
Location: W711, Block W, The Hong Kong Polytechnic University
Equipment-in-charge: Dr. Chi-Hang CHOW/ Sirius TSE (Office: TU503)
Email: chihang.chow@polyu.edu.hk / sirius.tse@polyu.edu.hk
Telephone: 3400 8813/ 3400 8878
