Removal of Endotoxin Using Amphiphilic Coreshell Nanosorbents

. MCSPTMToxin Clean – Highly Selective Endotoxin Removal .

The technology provides a fast and simple solution for selective removal of endotoxin from protein solutions in physiological buffers. It adopts a type of magnetic core-shell (MCS) composite particle, which can selectively bind endotoxin in protein mixtures and are easily removed by a magnetic separation. The endotoxin level of the treated protein solution is low enough (<10 EU/mL) to allow for further processing, analysis or even administration to mammals. The method has the following advantages:

• High selectivity for endotoxin adsorption in both basic and acidic protein mixtures, e.g. bovine serum albumin
• Effective in broad working conditions (different pHs, electrolyte concentrations and buffer types)
• Simple process which does not require multiple extraction steps (unlike Triton X-114 extraction method)
• Low cost, avoiding the use of expensive reagent, e.g. polylysine
• Fast purification process without common problems such as filter blocking in microfiltration and time-consuming process when using porous adsorbents