Proteomics in Eye Research

Quantitative Regulations of Protein. Tear biofluid proteomics.
Protein Biomarkers Discovery and Protein Translational Modifications in Ocular Diseases.

Proteomics has been regarded as the core research technology in the post-genomic era. Central to the life sciences in health discipline is the aim of identifying potential and small molecule biomarkers that may correlate to diseases, drug toxicity, genetic and environmental variations. Recent revolution in mass spectrometric (MS) platforms paves the way for rapid evaluation of potential biomarkers qualitatively and quantitatively for various diseases or physiological conditions. Cutting edge Next Generation MS platform can routinely allow thousands of unknown biological compounds be resolved and analyzed in an accurate and fully automated fashion. Ultimately, these measurements will provide novel insight into the biological regulation of signalling compounds in disease-causing alterations.

Using comprehensive proteomic line-up including discovery based and targeted based proteomics coupled with bioinformatics tools, we are interested in exploring the expressions / regulations of proteins in various ocular disorders and diseases (dry eyes, myopia, glaucoma and others)

Principal Investigator

Dr. Thomas LAM, Professor Chi-ho TO
School of Optometry

Representative Publication

J. Bian, Y.H. Sze, D.Y. Tse, C.H. To, S.A. McFadden, C.S. Lam, K.K. Li, T.C. Lam, SWATH Based Quantitative Proteomics Reveals Significant Lipid
Metabolism in Early Myopic Guinea Pig Retina, Int J Mol Sci 22(9) (2021).
Y.H. Sze, Q. Zhao, J.K.W. Cheung, K.K. Li, D.Y.Y. Tse, C.H. To, T.C. Lam*, High-pH reversed-phase fractionated neural retina proteome of
normal growing C57BL/6 mouse, Sci Data 8(1) (2021) 27.
F.J. Yu, T.C. Lam*, A.Y. Sze, K.K. Li, R.K. Chun, S.W. Shan, C.H. To, Alteration of retinal metabolism and oxidative stress may implicate myopic
eye growth: Evidence from discovery and targeted proteomics in an animal model, J Proteomics (2020) 103684.
S.W. Shan, D.Y. Tse, B. Zuo, C.H. To, Q. Liu, S.A. McFadden, R.K. Chun, J. Bian, K.K. Li, T.C. Lam*, Integrated SWATH-based and targetedbased proteomics provide insights into the retinal emmetropization process in guinea pig, J Proteomics 181 (2018) 1-15.
S.W. Shan, C.W. Do, T.C. Lam*, R.P.W. Kong, K.K. Li, K.M. Chun, W.D. Stamer, C.H. To, New Insight of Common Regulatory Pathways in Human
Trabecular Meshwork Cells in Response to Dexamethasone and Prednisolone Using an Integrated Quantitative Proteomics: SWATH and MRMHR Mass Spectrometry, Journal of proteome research 16(10) (2017) 3753-3765.