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Seminar - Magnetic-Assisted Rapid Aptamer Selection (MARAS) & New Biomedical Application Horizon of Aptamer by Prof. C.Y. Hong
日期:2016 年 07 月 22 日 ( 星期五)
Time:3:30 pm – 5:00 pm
Venue:EF305

Abstract:

Systematic Evolution of Ligands by EXponential enrichment (SELEX) was originally developed during the early 1990s. The SELEX protocol is an in vitro selection method for isolating DNA or RNA sequences. The basic steps of the SELEX process are incubation, separation, elution, amplification, and single-strand oligonucleotide purification; this is usually repeated for five to fifth selected rounds. Therefore, the aptamer generation using SELEX or its modified protocols is time consuming and technically difficult that limits the popularity of aptamer application. Recently, the Magnetic-Assisted Rapid Aptamer Selection (MARAS) method has been reported to generate high-binding affinity aptamers. The MARAS method does not require the repeated selection cycles to produce high-affinity aptamers and the generation of suitable aptamers using MARAS protocol becomes easy and rapid. Therefore, the new horizon in biomedical application using aptamers as capture ligands is emerged. In this talk, the new applications, including primer-free aptamer, multiplex immunoassay utilizing differential affinity and biomarker identification of disease, will be discussed and preliminary results will be presented.

Bio-sketch:

Prof. Hong was born in Chai-Yi, Taiwan, on December 1, 1950. In 1976, he received the B.S. degree in Aeronautical Engineering, Tamkung University, Taiwan. He then worked as a teaching assistant at the department from 1976 to 1977. He received his M.S. degree in Aeronautic and Astronautic Engineering from University of Illinois U.S.A., in 1979, and Ph.D. degree in 1984. Between 1979 to 1982 and 1984 to 1986, he worked as a senior engineering consultant in Chicago, U.S.A. Between 1986 to 1991, he worked as a senior engineer in McDonold Douglas Helicopter Company responsible for the development of helicopter rotor.

He returned to Taiwan and taught in Department of Mechanical Engineering, Da-Yeh University. He was promoted to full professorship 1999 and served as Dean of Research and Development of Da-Yeh University from 2004 to 2006. He moved to Nan-Kai University of Technology August 2007 and became a chair professor. Currently, he is a professor in Institute of Biomedical Engineering and a honorable distinguished professor in National Chung Hsing University, Taiwan. He has published more than 100 SCI journal papers and has more than 10 patents in the magnetic fluid research area. He has obtained a distinguished research award form National Science Council, Taiwan at 2003. His research interests include the basic properties and the applications of magnetic fluid, and magnetic nanoparticles on biomedical applications.